Identificação molecular de Candida spp. em amostras de pacientes usuários de próteses dentárias por meio de PCR - Multiplex

Abstract

Oral candidiasis is an opportunistic infection common to patients using dental prostheses. For the correct treatment is necessary the precise diagnosis of the yeast causing the infection since different species of Candida may be related. Thus, the objective of this study was to simultaneously identify different Candida species in samples of dental prosthesis patients by PCR - multiplex. The samples collected from dental prosthesis patients were inoculated in Saubourand medium and later the genomic DNA from the colonies was extracted by the organic extraction method with phenol-chloroform. The yeast DNA samples were amplified via two rounds of PCR. In the first one was used the primers ITS1 and ITS4 and in the second characterized as Multiplex with touchdown for amplification of molecular markers speciesspecific. The method used in the study was able to identify in a distinctive way the species C. albicans and C. troplicalis that colonized the oral cavity of the analyzed patient. Although, the multiplex PCR tested was able to identify the sensitivity was considered lower than expected. Thus, it is suggested that further studies can be performed in order to increase the efficiency of the test by promoting the standardization of the PCR reaction with increasing substances that raise the sensitivity and increase in the number of cycles in the thermal cycler.